 |
| Figure 1. Control plate contain no PGBR22 with LB Agar, Ampicillin, E. Coli BL21 (DE3), and SOC media showing no growth after approximately 27 hours of incubation in a 37 degrees Celsius incubator |
 |
| Figure 2. Experimental plate containing PGBR22, LB Agar, Ampicillin, E. Coli BL21 (DE3), SOC media with light purple colonies grown after approximately 27 hours of incubation in a 37 degrees Celsius incubator |
 |
| Figure 3. "Fun Plate" containing LB Agar, with tongue cells from each partner on each half of the petri dish after approximately 27 hours of incubation in a 37 degrees Celsius incubator |
 |
| Figure 4. Two 125 mL Erlenmeyer flasks containing LB Agar, Ampicillin, E. Coli BL21 (DE3), and PGBR22 after 24 hours in a shaking incubator operating at 37 degrees Celsius at 200-350 rpm. Turbid purple media indicates that bacteria grew. |
 |
| Figure 5. 50 mL conical tube containing LB Agar, Ampicillin, PGBR22, and E. Coli BL21 (DE3) with a total net weight of 62.08g after centrifuging for 10 minutes at 4 degrees Celsius and 5,000 rpm. Bacterial cells containing protein settled to the bottom. |
 |
| Figure 6. Elution 1 (left) and Elution 2 (right) consisting of PGBR22 after Ni-NTA chromatography. |
 |
| Figure 7. SDS Page gel placed on Whatman filter paper after being left overnight on the shaker. From left to right the wells consisted of the protein ladder, cell lysate, soluble fraction, flow through, wash buffer, Elution 1, and Elution 2 with the last three wells are being wash, Elution 1, and Elution 2. |
 |
| Figure 8. SDS Page Gel of PGBR22 placed on Whatman filter paper covered with cellophane after 1.5 hours of drying at 75 degrees Celsius on the Gradient cycle. From left to right the wells consisted of the protein ladder, cell lysate, soluble fraction, flow through, wash buffer, Elution 1, and Elution 2 with the last three wells are being wash, Elution 1, and Elution 2. |